| Product name | Calbindin D28K Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Calbindin D28K |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | CALB1; CAB27; Calbindin; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 30kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Calbindin encoded by CALB1 is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. |
| Gene ID | 793 |
| Alternative | CALB1; CAB27; Calbindin; Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type |
| Others | Calbindin D28K Polyclonal Antibody detects endogenous levels of Calbindin D28K protein. |
| Accession | P05937 |
| Observed Band(KD) | 30 |
Fig.1. Western Blot analysis of K562 cells using Calbindin D28K Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
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