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CA XII Polyclonal Antibody

CA XII Polyclonal Antibody

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Specification

Product name CA XII Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human CA XII at AA range: 200-280
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300;WB 1:500-1:2000;ELISA 1:40000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative CA12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 40kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. The gene product CA12 (carbonic anhydrase 12) is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for CA12.
Gene ID 771
Alternative CA12; Carbonic anhydrase 12; Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Tumor antigen HOM-RCC-3.1.3
Others CA XII Polyclonal Antibody detects endogenous levels of CA XII protein.
Accession O43570
Observed Band(KD) 39

Image & description

Fig. Western Blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1:2000.

Fig. Western Blot analysis of NIH-3T3 293T cells using CA XII Polyclonal Antibody diluted at 1:2000.

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