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C/EBP ε (phospho Thr74) Polyclonal Antibody

C/EBP ε (phospho Thr74) Polyclonal Antibody

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Specification

Product name C/EBP ε (phospho Thr74) Polyclonal Antibody
Immunogen Synthesized peptide derived from human C/EBP ε around the phosphorylation site of T74
Host Rabbit
Reactivity Human,Rat,Mouse,
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative CEBPE; CCAAT/enhancer-binding protein epsilon; C/EBP epsilon

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 34kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The CCAAT/enhancer binding protein epsilon encoded by CEBPE is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined.
Gene ID 1053
Alternative CEBPE; CCAAT/enhancer-binding protein epsilon; C/EBP epsilon
Others Phospho-C/EBP ε (T74) Polyclonal Antibody detects endogenous levels of C/EBP ε protein only when phosphorylated at T74.
Accession Q15744
Observed Band(KD) 34

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