| Product name | BID Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human BID around the non-phosphorylation site of S78 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | BID; BH3-interacting domain death agonist; p22 BID; BID |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 25kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | BID encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. BH3-interacting domain death agonist is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves BH3-interacting domain death agonist, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. |
| Gene ID | 637 |
| Alternative | BID; BH3-interacting domain death agonist; p22 BID; BID |
| Others | BID Polyclonal Antibody detects endogenous levels of BID protein. |
| Accession | P55957 |
Fig.1. Western Blot analysis of various cells using BID Polyclonal Antibody diluted at 1:1000.
Fig.2. Western Blot analysis of Jurkat cells using BID Polyclonal Antibody diluted at 1:1000.
You must be logged in to post a review.
Reviews
There are no reviews yet.