| Product name | Bcl-2 (phospho Thr56) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human Bcl-2 around the phosphorylation site of T56 |
| Host | Rabbit |
| Reactivity | Human,Rat,Mouse, |
| Applications | WB,IHC,IP,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | BCL2; Apoptosis regulator Bcl-2 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 26kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | BCL2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. |
| Gene ID | 596 |
| Alternative | BCL2; Apoptosis regulator Bcl-2 |
| Others | Phospho-Bcl-2 (T56) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at T56. |
| Accession | P10415 |
Fig. Western Blot analysis of various cells using Phospho-Bcl-2 (T56) Polyclonal Antibody diluted at 1:500.
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