| Product name | Bax Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Bax at AA range: 1-80 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | BAX; BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 22kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | The protein encoded by BAX belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein (BCL2 associated X, apoptosis regulator) forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for BAX. |
| Gene ID | 581 |
| Alternative | BAX; BCL2L4; Apoptosis regulator BAX; Bcl-2-like protein 4; Bcl2-L-4 |
| Others | Bax Polyclonal Antibody detects endogenous levels of Bax protein. |
| Accession | Q07812 |
Fig.1. Western Blot analysis of various cells using Bax Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of HeLa cells using Bax Polyclonal Antibody diluted at 1:2000.
Fig.3. Western Blot analysis of 293T using Bax Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000.
Fig.4. Western blot analysis of various cell Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000.
You must be logged in to post a review.
Reviews
There are no reviews yet.