| Product name | ATF-5 (Acetyl Lys29) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the human ATF-5 around the acetylation site of K29 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ATF5; ATFX; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 30kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | ATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. Activating transcription factor 5 binds the cAMP response element (CRE)(consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis. |
| Gene ID | 22809 |
| Alternative | ATF5; ATFX; Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx |
| Others | Acetyl-ATF-5 (K29) Polyclonal Antibody detects endogenous levels of ATF-5 protein only when acetylated at K29. |
| Accession | Q9Y2D1 |
Fig.1. Western Blot analysis of A549 cells using Acetyl-ATF-5(K29) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.
Fig.2. Western Blot analysis of A549 cells using Acetyl-ATF-5 (K29) Polyclonal Antibody. Secondary Antibody was diluted at 1:20000.
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