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Ataxin-2 Polyclonal Antibody

Ataxin-2 Polyclonal Antibody

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Specification

Product name Ataxin-2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human Ataxin-2 at AA range: 700-780
Host Rabbit
Reactivity Human,Mouse
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative ATXN2; ATX2; SCA2; TNRC13; Ataxin-2; Spinocerebellar ataxia type 2 protein; Trinucleotide repeat-containing gene 13 protein

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 140kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background ATXN2 belongs to a group of genes that is associated with microsatellite-expansion diseases, a class of neurological and neuromuscular disorders caused by expansion of short stretches of repetitive DNA. The protein (ataxin 2) encoded by ATXN2 has two globular domains near the N-terminus, one of which contains a clathrin-mediated trans-Golgi signal and an endoplasmic reticulum exit signal. The protein is primarily localized to the Golgi apparatus, with deletion of the Golgi and endoplasmic reticulum signals resulting in abnormal subcellular localization. In addition, the N-terminal region contains a polyglutamine tract of 14-31 residues that can be expanded in the pathogenic state to 32-200 residues. Intermediate length expansions of this tract increase susceptibility to amyotrophic lateral sclerosis, while long expansions of this tract result in spinocerebellar ataxia-2, an autosomal-dominantly inherited, neurodegenerative disorder. Alternative splicing results in multiple transcript variants.
Gene ID 6311
Alternative ATXN2; ATX2; SCA2; TNRC13; Ataxin-2; Spinocerebellar ataxia type 2 protein; Trinucleotide repeat-containing gene 13 protein
Others Ataxin-2 Polyclonal Antibody detects endogenous levels of Ataxin-2 protein.
Accession Q99700

Image & description

Fig.1. Western Blot analysis of various cells using Ataxin-2 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using Ataxin-2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293 cells using Ataxin-2 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of 293 cells using Ataxin-2 Polyclonal Antibody diluted at 1:1000.

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