| Product name | ARP Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human ARP |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | MANF; ARMET; ARP; Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 20kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Mesencephalic astrocyte-derived neurotrophic factor encoded by MANF is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. |
| Gene ID | 7873 |
| Alternative | MANF; ARMET; ARP; Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET |
| Others | ARP Polyclonal Antibody detects endogenous levels of ARP protein. |
| Accession | P55145 |
Fig.1. Western Blot analysis of 293 cells using ARP Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.
Fig.6. Immunohistochemical analysis of paraffin-embedded Mouse-brain, antibody was diluted at 1:100.
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