| Product name | Apelin Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Apelin |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:100-300;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | APLN; APEL; Apelin; APJ endogenous ligand |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 16kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | APLN encodes a peptide that functions as an endogenous ligand for the G-protein coupled apelin receptor. The encoded preproprotein is proteolytically processed into biologically active C-terminal peptide fragments. These peptide fragments activate different tissue specific signaling pathways that regulate diverse biological functions including fluid homeostasis, cardiovascular function and insulin secretion. This protein also functions as a coreceptor for the human immunodeficiency virus 1. |
| Gene ID | 8862 |
| Alternative | APLN; APEL; Apelin; APJ endogenous ligand |
| Others | Apelin Polyclonal Antibody detects endogenous levels of Apelin protein. |
| Accession | Q9ULZ1 |
Fig.1. Western Blot analysis of 22RV-1, H460, U87, Mouse brain cells using Apelin Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100.
Author：Serinkan Cinemre FB, Cinemre H, Bahtiyar N, Kahyaoğlu B, Ağaç MT, Shundo H, Sevinç L, Aydemir B Publication name：Ir J Med Sci IF：1.1
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