| Product name | AP-1 (phospho Thr239) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human AP-1 around the phosphorylation site of T239 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,IP,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 39-42kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | JUN is the putative transforming gene of avian sarcoma virus 17. It encodes Jun proto-oncogene, AP-1 transcription factor subunit which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. JUN is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. |
| Gene ID | 3725 |
| Alternative | JUN; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39 |
| Others | Phospho-AP-1 (T239) Polyclonal Antibody detects endogenous levels of AP-1 protein only when phosphorylated at T239. |
| Accession | P05412 |
Fig.1. Western Blot analysis of various cells using Phospho-AP-1 (T239) Polyclonal Antibody diluted at 1:500.
Fig.2. Western Blot analysis of HeLa cells using Phospho-AP-1 (T239) Polyclonal Antibody diluted at 1:500.
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