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Anti-His Tag Mouse Monoclonal Antibody (5C3)

Anti-His Tag Mouse Monoclonal Antibody (5C3)

Views(2409) Publications(26) Catalog no(ABT2050)
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Specification

Product name Anti-His Tag Mouse Monoclonal Antibody (5C3)
Immunogen Synthetic Peptide
Host Mouse
Reactivity Mammals, Bacteria
Applications IF, IP, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1500), IP (1:300).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Alternative His; 6 His epitope; Hexa His; HHHHHH epitope; Polyhistidine Tag

Image & description

Fig.1. Immunofluorescence staining (1:1500) of His fusion protein in 293 cells with red and counterstained with DAPI.

Fig.1. Immunofluorescence staining (1:1500) of His fusion protein in 293 cells with red and counterstained with DAPI.

Fig.2. Western blot analysis of 2ug His fusion protein with Anti-His Mouse Monoclonal Antibody (5C3) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).

Fig.2. Western blot analysis of 2ug His fusion protein with Anti-His Mouse Monoclonal Antibody (5C3) in 1:5000 dilution (lane A) and 1:10000 dilution (lane B).

Fig.3. IP (1:300) - WB (1:5000) analysis of His fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with His-tag protein (lane B); IP untransfected 293 cell lysate with Anti His tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti His tag mAb (lane E).

Fig.3. IP (1:300) - WB (1:5000) analysis of His fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with His-tag protein (lane B); IP untransfected 293 cell lysate with Anti His tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D) or with Anti His tag mAb (lane E).

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Tips for Abbkine antibodies

1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.

2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.

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