| Product name | Annexin V-APC/7-AAD Apoptosis Detection Kit |
| SampleType | Cells |
| Alternative | Annexin V |
| Kit components | • Annexin V Binding Buffer (5×) • Annexin V-APC •7-AAD •Apoptosis Positive Control Solution |
| Features & Benefits | • To streamline compensation for apoptosis detection, this kit is uniquely supplied with dedicated positive control cells •It employs a convenient one-step staining protocol, requiring only 15 minutes to complete •The kit demonstrates outstanding performance in distinguishing cell populations, enabling clear separation at different apoptotic stages and thereby greatly ensuring the accuracy of experimental data |
| Usage notes | • If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples. |
| Storage instructions | Stored at 4℃ for 12 months, protected from light |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. 7-AAD is a fluorescent nucleus dye, impermeant to live cells and apoptotic cells, but stains dead cells with red fluorescence, binding tightly to the nucleic acids in the cell. Annexin V-APC/7-AAD Apoptosis Detection kit provides a rapid and convenient assay for apoptosis. After staining a cell population with Annexin V-APC/7-AAD in the provided binding buffer, early apoptotic cells were only positive for Annexin V, dead cells were positive for Annexin V and 7-AAD, and live cells were negative for Annexin V and 7-AAD. In addition, this kit also provides Apoptosis Positive Control Solution specifically for apoptosis detection compensation regulation. Detection can be analyzed using flow cytometry or fluorescence microscopy |
| Alternative | Annexin V |
Fig.Annexin V-APC/7-AAD Apoptosis Detection Kit
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