| Product name | Amphiphysin II Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the C-terminal region of human Amphiphysin II |
| Host | Rabbit |
| Reactivity | Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | BIN1; AMPHL; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 64kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | BIN1 encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in several transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. |
| Gene ID | 274 |
| Alternative | BIN1; AMPHL; Myc box-dependent-interacting protein 1; Amphiphysin II; Amphiphysin-like protein; Box-dependent myc-interacting protein 1; Bridging integrator 1 |
| Others | Amphiphysin II Polyclonal Antibody detects endogenous levels of Amphiphysin II protein. |
| Accession | O00499 |
Fig.1. Western Blot analysis of Mouse brain cells using Amphiphysin II Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-pancreas, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.
Fig.5. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.
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