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ALDH2 Polyclonal Antibody

ALDH2 Polyclonal Antibody

Views(3) Publications(0) Catalog no(ABP53086)
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Specification

Product name ALDH2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human ALDH2
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:20000;IF 1:50-200
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative ALDH2; ALDM; Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 56kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Aldehyde dehydrogenase mitochondrial belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID 217
Alternative ALDH2; ALDM; Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI
Others ALDH2 Polyclonal Antibody detects endogenous levels of ALDH2 protein.
Accession P05091

Image & description

Fig.1. Western Blot analysis of A549 cells using ALDH2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western Blot analysis of A549 cells using ALDH2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.3. Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.

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