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AKAP 95 Polyclonal Antibody

AKAP 95 Polyclonal Antibody

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Specification

Product name AKAP 95 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human AKAP 95 at AA range: 300-380
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:20000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative AKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 76kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background AKAP8 encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. ThAKAP8 encodes a nuclear A-kinase anchor protein (A-kinase anchoring protein 8) that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of AKAP8 is located on the short arm of chromosome 9.
Gene ID 10270
Alternative AKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95
Others AKAP 95 Polyclonal Antibody detects endogenous levels of AKAP 95 protein.
Accession O43823

Image & description

Fig.1. Western Blot analysis of various cells using AKAP 95 Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using AKAP 95 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of MCF7 cells using AKAP 95 Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of MCF7 cells using AKAP 95 Polyclonal Antibody diluted at 1:1000.

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