| Product name | Actin-α cardiac muscle Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Actin-α cardiac muscle at AA range: 1-80 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:20000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 42kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. Actin alpha cardiac muscle 1 encoded by ACTC1 belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). |
| Gene ID | 70 |
| Alternative | ACTC1; ACTC; Actin, alpha cardiac muscle 1; Alpha-cardiac actin |
| Others | Actin-α cardiac muscle Polyclonal Antibody detects endogenous levels of Actin-α cardiac muscle protein. |
| Accession | P68032 |
Fig.1. Western Blot analysis of Mouse heart, Mouse lung cells using Actin-α cardiac muscle Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.
Fig.3. Immunohistochemical analysis of paraffin-embedded rat-muscle, antibody was diluted at 1:100.
Fig.4. Immunohistochemical analysis of paraffin-embedded Mouse-muscle, antibody was diluted at 1:100.
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