| Product name | Acetyl-Histone H3 (K9) Monoclonal Antibody (2E7) |
| Immunogen | Synthetic Peptide of Acetyl-Histone H3 (K9) |
| Host | Mouse |
| Reactivity | Human,Rat,Mouse |
| Applications | WB,IHC,IF |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-300;WB 1:500-2000;IF 1:50-200 |
| Clonality | Monoclonal |
| Preparation method | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Alternative | HIST1H3A |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 15kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. HIST3H3 (histone cluster 3 H3) is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. HIST3H3 is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
| Gene ID | 8290 |
| Alternative | HIST1H3A |
| Others | Acetyl-Histone H3 (K9) Monoclonal Antibody(2E7) detects endogenous levels of HIST1H3A. |
| Accession | P68431 |
Fig.1. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Acetyl Histone H3 K9 Mouse mAb diluted at 1:200.
Fig.2. Immunohistochemical analysis of paraffin-embedded Human Stomach Tissue using Acetyl Histone H3 K9 Mouse mAb diluted at 1:200.
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