| Product name | Mouse IL-5 Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IL-5 (Met 21-Asn 133) (P04401) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to induce TF-1 cells proliferation.The ED₅₀ for this effect is <0.2 ng/mL.The specific activity of recombinant mouse IL-5 is > 5 x 10⁶ IU/mg. |
| Protein length | The recombinant Mouse IL-5 Protein consists of 113 amino acids and predicts a molecular mass of 13.93 kDa |
| Preparation method | E. coli |
| Purity | > 98% as determined by SDS-PAGE. |
| Alternative | EDF, BCDFII, TRF |
| Formulation | The protein was lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 13.93 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | IL-5, also named B-cell differentiation factor I,eosinophil differentiation factor and TRF,is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3,IL-4,and granulocyte-macrophage colony-stimulating factor (GM-CSF),which are often co-expressed in TH2 cells.Through binding to the IL-5 receptor,IL-5 stimulates B cell growth and increases immunroglobulin secretion.It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma |
| Alternative | EDF, BCDFII, TRF |
| Accession | P04401 |
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