| Product name |
Mouse IL-1 beta Protein, His tag (Animal-Free) |
| Sequence |
Amino acid sequence derived from Mouse IL-1 beta (Val117-ser 229) (P10749) was expressed with 6×His tag at the C-terminus |
| Applications |
His tag |
| Applications notes |
Mouse |
| Activity |
Measured by its ability to induce D10.G4.1 cells proliferation.The ED50 for this effect is <8 pg/mL. |
| Protein length |
The recombinant Mouse IL-1 beta Protein consists of 113 amino acids and predicts a molecular mass of 18.3 kDa |
| Preparation method |
E. coli |
| Purity |
> 98% as determined by SDS-PAGE. |
| Alternative |
Catabolin, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) |
| Formulation |
The protein was Lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits |
Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight |
18.5 kDa |
| Usage notes |
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions |
Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping |
The product is shipped with blue ice. |
| Precautions |
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
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