| Product name | Human IL-8 (72 a.a.) Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Human IL-8 (Ser28-Ser99) (P10145) was expressed with 6×His tag at the C-terminus |
| Applications | His tag |
| Applications notes | Human |
| Activity | Measure by its ability to chemoattract PBMC.The ED₅₀ for this effect is <2 ng/mL. |
| Protein length | The recombinant Human IL-8 Protein consists of 72 amino acids and predicts a molecular mass of 9.32 kDa |
| Preparation method | E. coli |
| Purity | > 98% as determined by SDS-PAGE. |
| Alternative | Monocyte-Derived Neutrophil Chemotactic Factor (MDNCF), Neutrophil Activating Factor (NAF), CXCL8, C-X-C motif chemokine ligand 8 |
| Formulation | Lyophilized from sterile PBS, pH8.0 |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 9.32 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously. |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Human IL-8 (aa 28-99) predicts a molecular mass of 8.5 kDa and is the 72 amino acid residue form Ser 28 to Ser 99 of mature human IL-8. This recombinant protein is the major form secreted by macrophages. IL-8 is a chemokine produced by macrophages and other cell types such as epithelial cells, airway smooth muscle cells and endothelial cells. |
| Alternative | Monocyte-Derived Neutrophil Chemotactic Factor (MDNCF), Neutrophil Activating Factor (NAF), CXCL8, C-X-C motif chemokine ligand 8 |
| Accession | P10145 |
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