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Human IL-11 Protein, His tag (Animal-Free)

Human IL-11 Protein, His tag (Animal-Free)

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Specification

Product name Human IL-11 Protein, His tag (Animal-Free)
Sequence Amino acid sequence derived from Human IL-11 (Pro22-Leu199) (P20809) was expressed with 6×His tag at the N-terminus
Applications His tag
Applications notes Human
Activity Measure by its ability to induce T11 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human IL-11 is approximately >1 x 10⁷ IU/ mg.
Protein length The recombinant Human IL-11 Protein consists of 178 amino acids and predicts a molecular mass of 19.1 kDa
Preparation method E. coli
Purity > 98% as determined by SDS-PAGE.
Alternative AGIF (Adipogenesis Inhibitory Factor)

Product Properties

Formulation The protein was lyophilized from sterile PBS, pH 8.0. If you have any concerns or special requirements, please confirm with us.
Features & Benefits Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
Molecular weight 19.1 kDa
Usage notes Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously
Storage instructions Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles
Shipping The product is shipped with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Interleukin 11 (IL-11) is a cytokine, encoded by the gp130 family genes. IL-11 initially thought to be important for hematopoiesis, notably for megakaryocyte maturation, and it facilitate the formation of higher order structures involving dimers of gp130: IL-11:IL-11 RA complexes
Alternative AGIF (Adipogenesis Inhibitory Factor)
Accession P20809

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