| Product name | 14-3-3 ζ (phospho Ser58) Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from human 14-3-3 ζ around the phosphorylation site of S58 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 30kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for YWHAZ. |
| Gene ID | 7534 |
| Alternative | YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1 |
| Others | Phospho-14-3-3 ζ (S58) Polyclonal Antibody detects endogenous levels of 14-3-3 ζ protein only when phosphorylated at S58. |
| Accession | P63104 |
Fig.1. Western Blot analysis of various cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000.
Fig.2. Western Blot analysis of Jurkat cells using Phospho-14-3-3 ζ (S58) Polyclonal Antibody diluted at 1:2000.
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