| Product name | 14-3-3 β Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from the Internal region of human 14-3-3 β at AA range: 10-90 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications. |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1 |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 28kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | YWHAB encodes a protein (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta) belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for YWHAB. |
| Gene ID | 7529 |
| Alternative | YWHAB; 14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1 |
| Others | 14-3-3 β Polyclonal Antibody detects endogenous levels of 14-3-3 β protein. |
| Accession | P31946 |
Fig.1. Western Blot analysis of various cells using 14-3-3 β Polyclonal Antibody.
Fig.2. Western Blot analysis of Jurkat cells using 14-3-3 β Polyclonal Antibody.
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