Optimizing Human Myosin-VI Detection: A Practical Guide to the Abbkine KTE61414 ELISA Kit

Unlocking the Role of Myosin-VI in Cellular Dynamics​

Human Myosin-VI (MYO6), an unconventional motor protein, plays critical roles in clathrin-mediated endocytosis, maintenance of Golgi structure, and auditory function. Dysregulation links to cancers, hearing loss, and neurological disorders. Quantifying MYO6 levels is essential for elucidating its pathogenic mechanisms. However, conventional methods like Western blotting suffer from low throughput and semi-quantitative limitations. This underscores the need for sensitive, specific, and standardized tools – a gap addressed by advanced ELISA kits.

Introducing the Abbkine Human Myosin-VI (MYO6) ELISA Kit (KTE61414)​

The Abbkine Human Myosin-VI (MYO6) ELISA Kit (KTE61414)​ provides researchers with a robust solution for precise quantification of endogenous MYO6 in diverse biological samples. Engineered for performance, this kit leverages high-affinity antibodies validated against recombinant human MYO6. Its competitive format ensures specificity, minimizing cross-reactivity with other myosins or cellular proteins. For detailed specifications and ordering, visit the Abbkine product page.

Key Features Driving Research Efficiency​

• Superior Sensitivity:​ Detects MYO6 down to picogram levels (typically 15.6 - 1000 pg/mL range), capturing subtle expression changes crucial in disease models.
• Optimized Specificity:​ Antibody pairs rigorously tested to ensure minimal interference from related isoforms or sample matrix components.
• Streamlined Workflow:​ Complete kit includes pre-coated plates, standards, controls, detection reagents, and wash buffer. Achieve results within 3.5 hours, significantly faster than Western blot.
• Versatile Sample Compatibility:​ Validated for use with cell lysates, tissue homogenates, and potentially serum/plasma (user validation recommended), facilitating multi-assay studies.
• Rigorous Validation:​ Each lot undergoes stringent quality control testing, including spike-recovery and linearity assessments, ensuring batch-to-batch consistency.

Implementing the Kit: Best Practices for Reliable Results​

Maximizing the utility of the human myosin VI ELISA kit​ requires attention to detail:

1. Sample Prep is Paramount:​ Homogenize tissues thoroughly using appropriate lysis buffers containing protease inhibitors. Centrifuge lysates at high speed (e.g., 12,000 x g) to remove debris. Avoid repeated freeze-thaw cycles.
2. Dilution Strategy:​ Estimate target concentration based on preliminary data or literature. Use serial dilutions if necessary, ensuring signals fall within the standard curve's dynamic range. Include sample replicates (minimum duplicates).
3. Plate Layout & Controls:​ Utilize all wells systematically. Run multiple wells for standards and positive/negative controls provided in the KTE61414 ELISA kit. Include a blank well (sample diluent only) for background subtraction.
4. Incubation Timing & Temperature:​ Adhere strictly to recommended incubation times and temperatures (typically 37°C). Avoid deviations that can alter binding kinetics and affect accuracy.
5. Data Analysis:​ Generate a 4-parameter logistic (4-PL) standard curve using the provided software or compatible programs (e.g., GraphPad Prism, Excel add-ins). Ensure R² values exceed 0.99 for reliability. Report concentrations normalized to total protein content (BCA assay recommended) where applicable.

Why Choose Abbkine's MYO6 ELISA Solution?​

In a market saturated with varying quality ELISA offerings, the Abbkine Human Myosin-VI (MYO6) ELISA Kit (KTE61414)​ distinguishes itself through:

• Commitment to Quality:​ Abbkine's reputation rests on stringent manufacturing protocols and extensive validation data, providing confidence in every result obtained with their human MYO6 detection kit.
• Technical Support:​ Access to experienced scientists for protocol optimization queries or troubleshooting unexpected results is a significant advantage.
• Cost-Effectiveness:​ Delivers high-quality performance at a competitive price point compared to many premium alternatives, making longitudinal studies feasible.

Conclusion: Empowering MYO6 Research​

Accurate quantification of Human Myosin-VI is no longer a bottleneck. The Abbkine KTE61414 ELISA kit​ offers researchers a validated, sensitive, and efficient tool to advance investigations into MYO6's multifaceted biology. By following best practices for sample handling and assay execution, scientists can generate reliable data to drive discoveries in cancer biology, sensory neuroscience, and beyond. Explore the capabilities of this essential human myosin VI ELISA kit​ and elevate your research precision today via the Abbkine website.