A Premier Tool for His-Tagged Protein Analysis: Abbkine ABT2050 Anti-His Tag Mouse Monoclonal Antibody (5C3)

In the rapidly evolving landscape of molecular biology and protein research, the accurate detection, precise quantification, and detailed localization of His-tagged recombinant proteins stand as foundational pillars for advancing investigations in protein expression profiling, cellular signaling pathway dissection, structural biology characterization, and drug target validation. The Abbkine Anti-His Tag Mouse Monoclonal Antibody (5C3) (Cat. No.: ABT2050) emerges as a rigorously engineered, gold-standard solution, embodying the highest standards of specificity, application versatility, and experimental reproducibility to meet the stringent demands of modern scientific inquiry across academic, biotech, and pharmaceutical research settings.

The Significance of His Tag Antibodies in Advanced Research

Polyhistidine (His) tags—typically consisting of 6–10 consecutive histidine residues—are indispensable in recombinant protein technology due to their exceptional compatibility with both prokaryotic (e.g., E. coli) and eukaryotic (e.g., mammalian, insect) expression systems, minimal interference with protein folding, structure, and biological function, and efficient affinity purification via immobilized metal ion chromatography (IMAC). However, the reliability and robustness of downstream analytical workflows—including recombinant protein expression validation, subcellular localization mapping, protein-protein interaction elucidation, and functional activity assessment—hinge entirely on the performance characteristics of the anti-His tag antibody employed. Conventional antibodies often suffer from inherent limitations such as non-specific binding to endogenous proteins, restricted application scope, inconsistent binding affinity across experimental conditions, or batch-to-batch variability, all of which can lead to compromised data integrity, misleading results, and wasted research resources. Abbkine’s ABT2050 addresses these critical pain points through precision clone selection, advanced antibody engineering, and rigorous quality control, establishing a new benchmark for specificity, sensitivity, and versatility in His tag-targeted research tools.

Core Technical Advantages of ABT2050

• Superior Specificity and Sensitivity: As a mouse monoclonal antibody derived from the highly characterized 5C3 clone, ABT2050 exhibits exceptional binding affinity and stringent selectivity for His tags, ensuring negligible cross-reactivity with endogenous proteins, non-targeted biomolecules, or other affinity tags (e.g., GST, FLAG) across diverse sample matrices—including cell lysates, tissue homogenates, purified protein preparations, and conditioned media. Its high binding avidity enables robust detection and accurate quantification of low-abundance His-tagged proteins (down to picogram levels in many applications), facilitating reliable analysis even in samples with limited expression yields while minimizing experimental variability—a critical prerequisite for publishable, reproducible results in peer-reviewed research.
• Broad Species Reactivity: Meticulously optimized for consistent reactivity with both mammalian (e.g., human, mouse, rat) and bacterial (e.g., E. coli, Bacillus subtilis) samples, ABT2050 eliminates the need for researchers to source and validate multiple antibody reagents when transitioning between prokaryotic and eukaryotic expression systems. This cross-species compatibility enhances experimental flexibility, reduces workflow complexity, and ensures data consistency when comparing results across different model systems—whether investigating recombinant protein production in bacterial cultures or studying protein localization in mammalian cell lines.
• Multi-Application Compatibility: Rigorously validated for use in three core, high-impact research applications, ABT2050 provides a comprehensive, all-in-one solution for His-tagged protein characterization. It delivers exceptional performance in immunofluorescence (IF) and immunocytochemistry (ICC) for high-resolution subcellular localization studies, enabling researchers to visualize the spatial distribution of His-tagged proteins within cells with clear signal-to-noise ratios. In immunoprecipitation (IP), the antibody facilitates efficient pull-down of His-tagged proteins and their interacting partners, supporting downstream analyses such as mass spectrometry or Western Blot to unravel protein complex composition. Additionally, it excels in Western Blot (WB) applications for sensitive detection and semi-quantitative analysis of His-tagged protein expression levels, with consistent performance across reducing and non-reducing gel conditions. This multi-application versatility streamlines experimental workflows, reduces the time and cost associated with reagent sourcing, and ensures data coherence across complementary experimental approaches.
• Rigorous Quality Assurance and Cost-Effectiveness: Manufactured under ISO-compliant quality control protocols, each batch of ABT2050 undergoes extensive testing—including specificity assays against non-tagged proteins, sensitivity quantification via serial dilution experiments, stability testing under standard storage conditions, and lot-to-lot consistency verification—to ensure uncompromised technical performance. Priced at $79 for 50μl, the antibody delivers exceptional cost-effectiveness without sacrificing quality, making it accessible for academic research labs operating with limited budgets, biotech enterprises scaling up recombinant protein development, and pharmaceutical R&D teams conducting high-throughput screening campaigns.

Abbkine: A Trusted Partner in Life Science Research

With a proven track record of developing high-performance antibodies, proteins, biochemicals, and assay kits tailored to the needs of the global research community, Abbkine is committed to advancing scientific discovery through innovation, reliability, and accessibility. The Anti-His Tag Mouse Monoclonal Antibody (5C3) (ABT2050) reflects the brand’s dedication to addressing unmet research needs, leveraging cutting-edge antibody development technologies and rigorous quality control processes to deliver tools that combine technical excellence, user-centric design, and consistent performance. Backed by comprehensive technical support—including detailed application protocols, troubleshooting guides, and expert consultation—Abbkine ensures that researchers can maximize the value of their investment and achieve optimal results in every experiment.

Advance Your His-Tagged Protein Research

For researchers seeking a gold-standard antibody to elevate the rigor, reliability, and efficiency of their His-tagged protein studies, Abbkine ABT2050 offers unparalleled specificity, versatility, and reproducibility. To access detailed technical data sheets, application-specific protocols, batch validation reports, or to initiate a purchase, visit the official product page: https://www.abbkine.com/?s_type=productsearch&s=ABT2050

ABT2050 has emerged as the preferred choice for researchers aiming to eliminate experimental uncertainty and accelerate discovery in His-tagged protein research. By integrating this premier antibody into your workflow, you ensure that every experiment yields actionable, high-integrity data—propelling your research toward impactful scientific breakthroughs in protein biology, disease mechanism elucidation, and therapeutic development.