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WISP-3 Polyclonal Antibody

WISP-3 Polyclonal Antibody

Views(5) Publications(0) Catalog no(ABP57595)
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Specification

Product name WISP-3 Polyclonal Antibody
Immunogen Synthetic peptide from human protein at AA range: 1-50
Host Rabbit
Reactivity Human,Mouse
Applications WB,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000;ELISA 1:10000-20000
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative WNT1-inducible-signaling pathway protein 3; WISP-3; CCN family member 6

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 55kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background WISP3 (WNT1 inducible signaling pathway protein 3) encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. WISP3 is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of WISP3 are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis. Multiple transcript variants encoding different isoforms have been found for WISP3.
Gene ID 8838
Alternative WNT1-inducible-signaling pathway protein 3; WISP-3; CCN family member 6
Others The antibody detects endogenous WISP-3.
Accession O95389
Observed Band(KD) 55

Image & description

Fig.1. Western blot analysis of Hela Cell Lysate using antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western blot analysis of Hela Cell Lysate using antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.

Fig.2. Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200.

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