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Ribosomal Protein L27A Polyclonal Antibody

Ribosomal Protein L27A Polyclonal Antibody

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Specification

Product name Ribosomal Protein L27A Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human Ribosomal Protein L27A at AA range: 70-150
Host Rabbit
Reactivity Human,Mouse,Rat
Applications WB,IHC,IF,ELISA
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:40000;Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Alternative RPL27A; 60S ribosomal protein L27a

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 20kD
Storage buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL27A encodes a ribosomal protein that is a component of the 60S subunit. 60S ribosomal protein L27a belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.
Gene ID 6157
Alternative RPL27A; 60S ribosomal protein L27a
Others Ribosomal Protein L27A Polyclonal Antibody detects endogenous levels of Ribosomal Protein L27A protein.
Accession P46776
Observed Band(KD) 20

Image & description

Fig. Western Blot analysis of various cells using Ribosomal Protein L27A Polyclonal Antibody diluted at 1:2000.

Fig. Western Blot analysis of various cells using Ribosomal Protein L27A Polyclonal Antibody diluted at 1:2000.

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