| Product name | NOP56 Polyclonal Antibody |
| Immunogen | Synthesized peptide derived from NOP56 at AA range: 191-240 |
| Host | Rabbit |
| Reactivity | Human,Mouse,Rat |
| Applications | WB,IHC,IF,ELISA |
| Applications notes | Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000;IHC: 1:100-1:300;ELISA 1:10000;IF 1:50-200 |
| Clonality | Polyclonal |
| Preparation method | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Alternative | Nucleolar protein 56; Nucleolar protein 5A |
| Formulation | Liquid solution |
| Concentration | 1 mg/ml |
| Molecular weight | 65kD |
| Storage buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Storage instructions | Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Nop56p (NOP56 ribonucleoprotein) is a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Nop56p is required for assembly of the 60S ribosomal subunit and is involved in pre-rRNA processing. The protein encoded by NOP56 is similar in sequence to Nop56p and is also found in the nucleolus. Expansion of a GGCCTG repeat from 3-8 copies to 1500-2500 copies in an intron of this gene results in spinocerebellar ataxia 36. Multiple transcript variants encoding several different isoforms have been found for NOP56, but the full-length nature of most of them has not been determined. |
| Gene ID | 10528 |
| Alternative | Nucleolar protein 56; Nucleolar protein 5A |
| Others | NOP56 Polyclonal Antibody detects endogenous levels of NOP56. |
| Accession | O00567 |
| Observed Band(KD) | 65 |
Fig.1. Western Blot analysis of HEPG2 Hela cells using NOP56 Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.
Fig.2. Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.
Fig.3. Immunohistochemical analysis of paraffin-embedded human-placenta, antibody was diluted at 1:200.
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