Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. hTERT (TERT) is the rate-limiting catalytic subunit of telomerase. The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks.
Abbkine TERT Polyclonal Antibody was affinity-purified from rabbit serum by affinity-chromatography using synthetic peptide from human protein at AA range: 1268-1341. The verified reactivity and applications respectively are human and ELISA, IHC-p, WB. Abbkine suggested the starting dilutions are as follows: WB: 1:500-1:2000, IHC-p: 1:50-1:300, ELISA 1:5000-1:20000.
Supplied as liquid solution at 1 mg/ml, the antibody is stable for one year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.