PurKine™ His-Tag Ni-NTA Resin 6FF effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.
Abbkine PurKine™ His-Tag Ni-NTA Resin 6FF is stored in PBS buffer containing 20% ethanol. The Resin can be used in native and denaturing conditions and more than 50 mg of 6xHis-tagged protein would be captured per milliliter of resin. No performance decrease after at least five repeated uses of the same batch of resin. In addition, the highly crosslinked beads tolerate linear flow rates are up to 500 cm/hour.
In my opinion," FF " means "fast flow". Because of the high cross-link of PurKine™ His-Tag Ni-NTA Resin 6FF, the maximum pressure it can afford is 0.3MPa, 3bar and the tolerate linear flow rate is up to 500 cm/hour. It's very suitable for industry customers like me who demands large-scale production. This product fully comply with my request. If you're working in the laboratory, I suggest you purchase PurKine™ His-Tag Ni-NTA Packed Column 6FF (Resin is prepacked in the column ).