Protein At is an alkali-resistant protein A (Alkaline tolerate) based on the native protein A though biotechnology mutation. Protein At Resin has been immobilized for the capture of monoclonal antibodies at process scale. Protein At Resin provides greater stability than conventional protein A-based resin in alkaline conditions used in cleaning-in-place (CIP) . The enhanced alkali stability of protein At Resin improves process economy and product quality.
PurKine™ Protein AT (alkaline tolerate) Resin 4FF consists of 90μm beads of cross-linked 4% agarose, to which Recombinant protein A (alkaline tolerate) has been coupled. The resin withstands rigorous CIP and sanitization procedures with 0.1 to 0.5 M NaOH. The resin load is almost unchanged after hundreds of CIP. At mean time, it's a robust, high-flow resin that ensures excellent pressure/flow properties, low non-specific binding , and an oriented ligand coupling for optimal binding capacity.
The binding capacity of PurKine™ Protein AT (alkaline tolerate) Resin 4FF is more than 40mg human lgG for per ml of resin. Tests confirm the resin has an increased stability towards proteases, leading to lower ligand leakage. For large-scale industry production, Protein AT (alkaline tolerate) Resin 4FF maybe your best choice as CIP can be performed with cost-effective reagents such as sodium hydroxide instead of expensive and hazardous cleaning agents such as Gua-HCl.