Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to the Fc region of immunoglobulins through interactions with the heavy chain. For antibody affinity purification, recombinant protein A is a better choice. Recombinant protein A is produced in E. coli that contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding.
Abbkine PurKine™ Protein A Resin 4FF has been immobilized for use in IgG purification applications at a variety of scales. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Recombinant protein A has been coupled. Per mL of resin can capture at least 40mg human lgG. In addition, its high flow properties (up to 300cm/hour) make it excellent for scaling-up.
PurKine™ Protein A Purification system is designed for easy, one-step purification of antibody from ascites fluid, serum (polyclonal antibody), or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein A Resin 4FF is more suitable for large-scale industry purification to save time as well as energy. It can combine with Peristaltic pump or AKTA instrument .