PurKine™ His-Tag Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins. The Resin consists of 90μm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). PurKine™ His-Tag Ni-NTA Resin has extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions.
Abbkine's PurKine™ His-Tag Ni-NTA Resin is stored in PBS buffer containing 20% ethanol. The Resin can be used in native and denaturing conditions and more than 50 mg of 6xHis-tagged protein would be captured per milliliter of resin. No performance decrease after at least five repeated uses of the same batch of resin.
I have used Abbkine's PurKine™ His-Tag Ni-NTA Resin for five times, and I don't regenerate the Ni-NTA so far. The resin shows very high batch-to-batch reproducibility , binding capacity and chemical stability. Our laboratory also purchased PurKine™ His-Tag Ni-NTA Packed Column and PurKine™ His-Tag Protein Purification Kit (Ni-NTA), and I haven't heard any complaints about the products until now.