FLAG-tag, is a polypeptide tag that can be added to a protein using recombinant DNA technology, having the sequence DYKDDDDK. It has been used for studying proteins in living cells and for protein purification by affinity chromatography. It has been used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. Because of the small size of the FLAG peptide tag, it is not likely to obscure other epitopes, domains, or alter function, secretion, or transport of the fusion protein. The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da).
PurKine™ Anti-Flag Resin 4FF has been designed for affinity purification of Flag fusion proteins.The Resin consists of 90μm beads of cross-linked 4% agarose, to which mouse monoclonal antibody against DYKDDDDK tag has been coupled. Tests confirm that performance equals or exceeds popular Anti-Flag resins from other suppliers, and no decrease in performance occurs after at least five repeated uses. In addition, its high flow properties make it excellent for scaling-up.
More than 1mg DYKDDDDK-tagged protein can be captured by per mL of PurKine™ Anti-Flag Resin 4FF. The maximum tolerance pressure of the resin is 0.3MPa, 3bar, which is excellent for industry production. In addition, Anti-Flag Tag Mouse Monoclonal Antibody (1B10) is a good option to detect the Flag fusion protein that is affinity purified.