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Vimentin Monoclonal Antibody

Vimentin Monoclonal Antibody

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Specification

Product name Vimentin Monoclonal Antibody
Immunogen Recombinant Protein
Host Mouse
Reactivity Human, Mouse, Rat
Applications WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:1000-1:3000).
Clonality Monoclonal
Preparation method The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Alternative VIM; Vimentin

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 50-57 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background VIM encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in VIM causes a dominant, pulverulent cataract.
Gene ID 7431
Alternative VIM; Vimentin
Others The antibody detects endogenous Vimentin protein.
Accession P08670

Image & description

Fig.1. Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.

Fig.1. Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.

Fig.2. Western blot analysis of 293T cell with Mouse mAb diluted at 1:1000.

Fig.2. Western blot analysis of 293T cell with Mouse mAb diluted at 1:1000.

Fig.3. The picture was kindly provided by our customer.

Fig.3. The picture was kindly provided by our customer.

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