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Tubulin α Polyclonal Antibody

Tubulin α Polyclonal Antibody

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Specification

Product name Tubulin α Polyclonal Antibody
Immunogen Synthesized peptide derived from part region of human Tubulin α protein at amino acid sequence of 382-451
Host Rabbit
Reactivity Human, Mouse, Rat
Applications ELISA, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000-1:20000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen
Alternative Tubulin alpha-1A chain; TUBA1A; TUBA3; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain;

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. TUBA1A encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. TUBA1A is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in TUBA1A cause lissencephaly type 3 (LIS3)-a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID 7846
Alternative Tubulin alpha-1A chain; TUBA1A; TUBA3; Alpha-tubulin 3; Tubulin B-alpha-1; Tubulin alpha-3 chain;
Others The antibody detects endogenous levels of Tubulin α
Accession Q71U36/P68363
Observed Band(KD) 55

Image & description

Fig.1. Western blot analysis of Hela lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

Fig.1. Western blot analysis of Hela lysate, antibody was diluted at 1:2000. HRP, Goat Anti-Rabbit IgG (Cat #: A21020) secondary antibody was diluted at 1:20000.

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