TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution)

Product Information

  • Product name

    TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution)

  • Application notes

    TraKine™ Pro is series of long-term super-resolution cell staining imaging portfolio for labeling subcellular structures of live and fixed cells. TraKine™ Pro proprietary excellent fluorescent dyes span the full UV-visible and near IR spectrum. Customized products based on TraKine™ Pro technology are also available. TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution) is a fluorescence imaging tool for staining of Tubulin in mammalian living cells with high specificity and low background. The proprietary probe in the kit consists of a microtubule recognition unit and a deep red fluorescent dye (Ex/Em=650/665 nm), the recognition unit can selectively recognize microtubules and binding with it. The whole probe is membrane impermeable, but after incubating with buffer T, it can enter cells through endocytosis pathway in vesicular compartments, and release into cytoplasm subsequently. It is especially suitable for long-term super-resolution imaging.

Fig.1. SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution). The filamentous structure of microtubules can be clearly labeled.

Fig.2. Confocal imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.

Fig.3. Confocal imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.

Product Properties

  • Kit components

    • TubRed™ (200 uM) • Buffer T

  • Features & Benefits

    • Optimized staining protocol for labeling Tubulin in mammalian living cells. • Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, TIRF, STORM and PALM). • Proprietary TubRed™ (Ex/Em = 650/665 nm)-high specificity, low background and excellent photostability. • Low levels of cytotoxicity.

  • Usage notes

    Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.

  • Storage instructions

    Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology. The most studied tubulins have been isolated from vertebrate brains. The microtubules can be viewed in immunofluorescent microscopy, which enables the observation of the intracellular organization of proteins that are in the form of a supramolecular structure.

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TraKine™ Pro Live-cell Tubulin Staining kit (Deep Red Fluorescence with Super Resolution)
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTC4110

  • Size
  • Price
  • 50T $180
  • 250T $620
  • 1000T $2224

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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