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TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution)

Product Information

  • Product name

    TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution)

  • Application notes

    TraKine™ Pro is series of long-term super-resolution cell staining imaging portfolio for labeling subcellular structures of live and fixed cells. TraKine™ Pro proprietary excellent fluorescent dyes span the full UV-visible and near IR spectrum. Customized products based on TraKine™ Pro technology are also available. TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution) is a fluorescence imaging tool for staining of Lysosome in mammalian living and fixed cells with high specificity and low background. The proprietary probe in the kit consists of a lysosome recognition unit and a deep red fluorescent dye (Ex/Em: 650/665nm), which has high fluorescence quantum yield and high photostability. The probe can selectively recognize and label lysosome. It is especially suitable for long-term super-resolution imaging.

Fig.1. SIM imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution). It can be seen that the kit can specifically label Lysosome structures in living cells and perform ultra-high resolution imaging.

Fig.2. Confocal imaging results of U2OS cells using Abbkine TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution). It shows that the kit has high labeling efficiency.

Fig.3. STORM imaging results of BSC-1 cells using Abbkine TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution).

Product Properties

  • Kit components

    • LysRed™ (200 uM)
    • Buffer L

  • Features & Benefits

    • Optimized staining protocol for labeling Lysosome in mammalian living and fixed cells.
    • Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, TIRF, STORM and PALM).
    • Proprietary LysRed™ (Ex/Em = 650/665nm)-high specificity, low background and excellent photostability.
    • Low levels of cytotoxicity.

  • Usage notes

    Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment.

  • Storage instructions

    Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    Extensive research has indicated that intracellular organelles are integrated into cellular networks and collaborate on various cellular tasks rather than acting as isolated entities. Specialized membrane contact sites are formed between organelles, providing distinct spatial regions for executing and regulating their functions. Lysosomes and mitochondria are crucial dynamic organelles that participate extensively in many important cellular processes. Their dysfunction has been implicated in diverse diseases such as neurodegenerative disorders, cancer, and cardiovascular diseases.

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TraKine™ Pro Live-cell Lysosome Staining kit (Deep Red Fluorescence with Super Resolution)
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact service@abbkine.com for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through support@abbkine.com if you need more information or support.

Cat #: KTC4210

  • Size
  • Price
  • 50T $160
  • 250T $590
  • 1000T $1720

Contact information

Do not hesitate to contact us if you have any questions.

  •  Customer: service@abbkine.com
  •  Message: Click to Leave
  •  Tel: +86-27-59716789
  •  Fax: +86-27-59716788
  • Global Headquarters: Bldg 1, Harbour of Technology Times, No.35, Optical Valley Ave, Wuhan, Hubei, China. 430074

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