| Product name | TraKine™ Pro Live-cell Lyso-traker kit (Green Fluorescence) |
| Applications notes | TraKine™ Pro is series of long-term super-resolution cell staining imaging portfolio for labeling subcellular structures of live and fixed cells. |
| Kit components | •Lyso Green (200 μM) •Buffer A (200 μM) |
| Features & Benefits | • Optimized staining protocol for labeling Lysosome in mammalian living and fixed cells. The product has been tested in U2OS, Hela, Cos-7 and ARPE cell lines. If the sample type is not included in the above cell lines, we can provide experimental services for specific cell lines. • Especially suitable for Confocal and long-term super-resolution imaging (such as SIM, STED, TIRF, and PALM). • Proprietary MitoRed™ (Ex/Em = 647/661nm)-high specificity, low background and excellent photostability. • Low levels of cytotoxicity. |
| Usage notes | Make sure the pipette tips and PCR tubes were sterilized at high temperature and pressure. Make sure sterile environment and protect from light during the whole experiment. • To avoid MitoRed™ adsorbed on glass that may affect the signal to noise ratio. Nunc Glass Bottom Dishes is recommended for better image quality. |
| Storage instructions | Refer to list of materials supplied for storage conditions of individual components. Stable for at least 6 months at recommended temperature from date of shipment. |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Background | Extensive research has indicated that intracellular organelles are integrated into cellular networks and collaborate on various cellular tasks rather than acting as isolated entities. Specialized membrane contact sites are formed between organelles, providing distinct spatial regions for executing and regulating their functions. Lysosomes and mitochondria are crucial dynamic organelles that participate extensively in many important cellular processes. Their dysfunction has been implicated in diverse diseases such as neurodegenerative disorders, cancer, and cardiovascular diseases. |
Fig.1.Long time series images (a, c) and corresponding fluorescence bleaching curves (b, d) were obtained by using HiS SIM Abbkine TraKine™ Pro Live-cell Lyso-traker kit (Green Fluorescence)and Spining disk confocal microscopy of Tubulin Green and commercially available Lyso Tracker Green on U-2 OS cells, respectively. Scale bars: 5 μM.
Author：L Hu, C Dong, Z Wang, S He, Y Yang, M Zi, H Li Publication name：Aging Cell IF：7.8
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1.The species of antibody reactivity should be the sample species that can be matched normally after Abbkine R&D experts have passed strict scientific verification. If your sample is not within the range of reactivity, in order to improve the efficiency and results of your experiment, it is not suggested to try other species. Otherwise, it may lead to sample mismatch and affect the effect of your experiment.
2.Please aliquot the antibody received as soon as possible and store it at -20℃, avoid repeated freezing and thawing, and use it within one year.
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