Login Register
English
0

Cart

$ 0

Topo IIβ Polyclonal Antibody

Topo IIβ Polyclonal Antibody

Views(190) Publications(0) Catalog no(ABP56420)
Datasheet Print Share Email Share
  • Specification
  • FAQ
  • Publications(0)
  • Comments(0)

Specification

Product name Topo IIβ Polyclonal Antibody
Immunogen Synthesized peptide derived from the N-terminal region of human Topo IIβ at AA range: 10-90
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative TOP2B; DNA topoisomerase 2-beta; DNA topoisomerase II; beta isozyme

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TOP2B (topoisomerase (DNA) II beta) encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. TOP2B encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in TOP2B have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of TOP2B results in two transcript variants; however, the second variant has not yet been fully described.
Gene ID 7155
Alternative TOP2B; DNA topoisomerase 2-beta; DNA topoisomerase II; beta isozyme
Others Topo IIβ Polyclonal Antibody detects endogenous levels of Topo IIβ protein.
Accession Q02880
Observed Band(KD) 183

Image & description

This product has been cited in0publications

Reviews

There are no reviews yet.

Be the first to review “Topo IIβ Polyclonal Antibody”