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Topo IIα Polyclonal Antibody

Topo IIα Polyclonal Antibody

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Specification

Product name Topo IIα Polyclonal Antibody
Immunogen Synthesized peptide derived from human Topo IIα around the non-phosphorylation site of S1213
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
Gene ID 7153
Alternative TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme
Others Topo IIα Polyclonal Antibody detects endogenous levels of Topo IIα protein.
Accession P11388
Observed Band(KD) 174

Image & description

Fig. Western Blot analysis of extracts from K562 cells, using Topo IIα Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig. Western Blot analysis of extracts from K562 cells, using Topo IIα Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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