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TNF α Polyclonal Antibody

TNF α Polyclonal Antibody

Views(388) Publications(0) Catalog no(ABP57281)
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Specification

Product name TNF α Polyclonal Antibody
Immunogen Recombinant Protein
Host Rabbit
Reactivity Human, Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:50-1:200).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background TNF (tumor necrosis factor) encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.
Gene ID 7124
Others The antibody detects TNF α.
Accession P01375
Observed Band(KD) 17,25

Image & description

Fig.1. Western blot analysis of SH-SY5Y 293T 3T3 lysis using TNF α antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western blot analysis of SH-SY5Y 293T 3T3 lysis using TNF α antibody. Antibody was diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western blot analysis of Recombinant Human TNF a Protein with TNF α Rabbit pAb diluted at 1:2000.

Fig.2. Western blot analysis of Recombinant Human TNF a Protein with TNF α Rabbit pAb diluted at 1:2000.

Fig.3. Western blot analysis of LPS Treated HepG2 with TNF α Rabbit pAb diluted at 1:2000.

Fig.3. Western blot analysis of LPS Treated HepG2 with TNF α Rabbit pAb diluted at 1:2000.

Fig.4. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using TNF aRabbit pAb diluted at 1:200.

Fig.4. Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using TNF aRabbit pAb diluted at 1:200.

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