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TIF1α Polyclonal Antibody

TIF1α Polyclonal Antibody

Views(13) Publications(0) Catalog no(ABP52608)
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Specification

Product name TIF1α Polyclonal Antibody
Immunogen Synthesized peptide derived from the C-terminal region of human TIF1α at AA range: 960-1040
Host Rabbit
Reactivity Human, Mouse
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative TRIM24; RNF82; TIF1; TIF1A; Transcription intermediary factor 1-alpha; TIF1-alpha; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Transcription intermediary factor 1-alpha encoded by TRIM24 mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Gene ID 8805
Alternative TRIM24; RNF82; TIF1; TIF1A; Transcription intermediary factor 1-alpha; TIF1-alpha; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24
Others TIF1α Polyclonal Antibody detects endogenous levels of TIF1α protein.
Accession O15164
Observed Band(KD) 117

Image & description

Fig.1. Western Blot analysis of various cells using TIF1α Polyclonal Antibody diluted at 1:1000.

Fig.1. Western Blot analysis of various cells using TIF1α Polyclonal Antibody diluted at 1:1000.

Fig.2. Western Blot analysis of COLO205 cells using TIF1α Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Western Blot analysis of COLO205 cells using TIF1α Polyclonal Antibody diluted at 1:1000. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

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