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TERT Polyclonal Antibody

TERT Polyclonal Antibody

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Specification

Product name TERT Polyclonal Antibody
Immunogen Synthetic peptide from human protein at AA range: 1268-1341
Host Rabbit
Reactivity Human
Applications ELISA, IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:200-1:500), ELISA (1:10000-1:20000).
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen
Alternative Telomerase reverse transcriptase (EC 2.7.7.49) HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2; TP2

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Molecular weight 126 KD
Storage buffer PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background Telomerase is a ribonucleoprotein polymerase that maintains telomere ends by addition of the telomere repeat TTAGGG. The enzyme consists of a protein component with reverse transcriptase activity, encoded by TERT (telomerase reverse transcriptase), and an RNA component which serves as a template for the telomere repeat. Telomerase expression plays a role in cellular senescence, as it is normally repressed in postnatal somatic cells resulting in progressive shortening of telomeres. Deregulation of telomerase expression in somatic cells may be involved in oncogenesis. Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. Alternatively spliced variants encoding different isoforms of telomerase reverse transcriptase have been identified; the full-length sequence of some variants has not been determined. Alternative splicing at this locus is thought to be one mechanism of regulation of telomerase activity.
Gene ID 7015
Alternative Telomerase reverse transcriptase (EC 2.7.7.49) HEST2; Telomerase catalytic subunit; Telomerase-associated protein 2; TP2
Others The antibody detects endogenous TERT.
Accession O14746

Image & description

Fig.1. Immunohistochemical analysis of paraffin-embedded human kidney tissue. 1, TERT Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.1. Immunohistochemical analysis of paraffin-embedded human kidney tissue. 1, TERT Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.

Fig.2. Western blot analysis of hepg2 Cell Lysate, antibody was diluted at 1:1000. secondary antibody was diluted at 1:20000.

Fig.2. Western blot analysis of hepg2 Cell Lysate, antibody was diluted at 1:1000. secondary antibody was diluted at 1:20000.

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