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SuperLumia ECL Plus HRP Substrate Kit

Product Information

  • Product name

    SuperLumia ECL Plus HRP Substrate Kit

  • Application notes

    For best results, it is essential to optimize all system components including sample amount, antibody concentrations and the membrane and blocking reagents. SuperLumia ECL Reagent is extremely sensitive and requires much less sample and antibodies than most commercially available substrates.

Fig.Abbkine SuperLumia ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns.

Product Properties

  • Kit components

    • ECL HRP Substrate Reagent A • ECL HRP Substrate Reagent B.

  • Features & Benefits

    Abbkine SuperLumia ECL Plus HRP Substrate Kit is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

  • Usage notes

    1.Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution.2.Suggest 0.1-0.2ml of SuperLumia ECL Plus working solution for 1 cm2 size of blot membrane. 3.Incubate blot 1-5 minutes in SuperLumia ECL Plus Substrate working solution.4.Drain excess reagent. Cover blot with clear plastic wrap.5.Expose blot to X-ray film.

  • Storage instructions

    Stable for one year at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.

  • Shipping

    Gel pack with blue ice.

  • Precautions

    The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

  • Background

    The family of SuperLumia ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. SuperLumia ECL Plus chemiluminescent HRP substrate reagent is specially optimized for femtogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of femtogram amounts of antigen with photographic or other imaging methods perfectly well.

  • Alternative names

    Enhanced Chemiluminescence; ECL; Electrochemiluminescence


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SuperLumia ECL Plus HRP Substrate Kit
Click to download booklet for more details

Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact for your enquiry.

Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.

Preparation methods for Biochemical

Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.

  1. ♦ What and how to make a "good" buffer?

Antibody application protocols

Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.

  1. ♦ Antibody Western Blotting (WB) protocol
  2. ♦ Antibody Immunohistochemistry (IHC) protocol
  3. ♦ Antibody Immunofluorescence (IF) protocol
  4. ♦ Antibody Immunoprecipitation (IP) protocol
  5. ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol

Protein&peptide usage suggestions

Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.

  1. ♦ Handling and storage suggestion for peptides and protein
  2. ♦ Cytokines and growth factors for cell culture application

Commonly used assay kits guidelines

Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.

We hope this will be helpful for your research work. Please let us know through if you need more information or support.

  1. Synthesis and discovery of new compounds bearing coumarin scaffold for the treatment of pulmonary fibrosis

    D Deng. European Journal of Medicinal Chemistry

  2. A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation

    CX Zhang, XW Zhu, LL Li, TK Ma, M Shi.Diabetes Metabolic Syndrome and Obesity. 2019.

  3. The therapeutic potential of GABA in neuron-glia interactions of cancer-induced bone pain

    Meng-Meng Ge, Shu-Ping Chen, Ya-Qun Zhou, et al. Eur J Pharmacol. 2019 Jun 19;858:172475.

  4. Discovery of human TyrRS inhibitors by structure-based virtual screening, structural optimization, and bioassays

    Shenzhen Huang, Xiang Wang, Guifeng Lin, et al. royal society of chemistry

  5. Effect of porous tantalum on promoting the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro through the MAPK/ERK signal pathway

    Xiaojie Dou, Xiaowei Wei, Ge Liu, et al. Journal of Orthopaedic Translation.

  6. Histamine-4 receptor antagonist JNJ7777120 inhibits pro-inflammatory microglia and prevents the progression of Parkinson-like pathology and behaviour in a rat model

    Pei Zhou, Judith R. Homberg, Qiuyuan Fang, et al. Brain Behav Immun. 2019 Feb;76:61-73.

  7. Sinomenine attenuates cancer-induced bone pain via suppressing microglial JAK2/STAT3 and neuronal CAMKII/CREB cascades in rat models

    Shu-Ping Chen, Jia Sun, et al. Molecular Pain. 2018; 14: 1-14.

  8. SKLB-23bb, A HDAC6-Selective Inhibitor, Exhibits Superior and Broad-Spectrum Antitumor Activity via Additionally Targeting Microtubules

    Wang F, Zheng L, Yi Y, et al. Mol Cancer Ther. 2018, 17(4): 763-775.

  9. Hypoxia-inducible factor-1α activates transforming growth factor-β1/Smad signaling and increases collagen deposition in dermal fibroblasts

    X MY, P QQ, X SQ, et al. Oncotarget, 2017, 9(3): 3188-3197.

  10. Construction, immune protection and innate immune response of shuffled polyvalent ompAs vaccines

    Wang S N, Cheng Z X, Ling P, et al. Fish Shellfish Immunol, 2018, 74: 325-331.

  11. Endoplasmic Reticulum Stress in Spinal Cord Contributes to the Development of Morphine Tolerance

    Liu D, Zhou Y, Peng Y, et al. Front Mol Neurosci, 2018, 6; 11: 72.

  12. Six genes of ompA family shuffling for development of polyvalent vaccines against Vibrio alginolyticus and Edwardsiella tarda

    Cheng Z X, Chu X, Wang S N, et al. Fish Shellfish Immunol, 2018, 75: 308-315.

  13. The Role of Spinal GABAB Receptors in Cancer-Induced Bone Pain in Rats

    Zhou, Ya-Qun, et al. The Journal of Pain (2017).

  14. p8 attenuates the apoptosis induced by dihydroartemisinin in cancer cells through promoting autophagy

    Chen, Sang-Sang, et al. Cancer biology & therapy 16.5 (2015): 770-779.

  15. Development of the photosynthetic apparatus of Cunninghamia lanceolata in light and darkness.

    Xue X, Wang Q, Qu Y, et al. New Phytologist, 2016.

  16. Electroacupuncture relieves labour pain and influences the spinal dynorphin/κ-opioid receptor system in rats.

    Jiang Q Y, Wang M Y, Li L, et al. Acupuncture in Medicine, 2016, 34(3): 223-228.

Cat #: K22030

  • Size
  • Price
  • 30ml $45
  • 100ml $110
  • 200ml $210
  • 500ml $440

Contact information

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