Product Information

• Product name

  SuperLumia ECL Plus HRP Substrate Kit

• Application notes

  For best results, it is essential to optimize all system components including sample amount, antibody concentrations and the membrane and blocking reagents. SuperLumia ECL Reagent is extremely sensitive and requires much less sample and antibodies than most commercially available substrates.

Fig.Abbkine SuperLumia ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns.

Product Properties

• Kit components

  • ECL HRP Substrate Reagent A
  • ECL HRP Substrate Reagent B.

• Features & Benefits

  Abbkine SuperLumia ECL Plus HRP Substrate Kit is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

• Usage notes

  1.Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution. 2.Suggest 0.1-0.2ml of SuperLumia ECL Plus working solution for 1 cm2 size of blot membrane. 3.Incubate blot 1-5 minutes in SuperLumia ECL Plus Substrate working solution. 4.Drain excess reagent. Cover blot with clear plastic wrap. 5.Expose blot to X-ray film.

• Storage instructions

  Stable for one year at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.

• Shipping

  Gel pack with blue ice.

• Precautions

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

• Background

  The family of SuperLumia ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. SuperLumia ECL Plus chemiluminescent HRP substrate reagent is specially optimized for femtogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of femtogram amounts of antigen with photographic or other imaging methods perfectly well.

• Alternative names

  Enhanced Chemiluminescence; ECL; Electrochemiluminescence