- Product name
SuperLumia ECL HRP Substrate Kit
- Application notes
For best results, it is essential to optimize all system components including sample amount, antibody concentrations and the membrane and blocking reagents. SuperLumia ECL Reagent is extremely sensitive and requires much less sample and antibodies than most commercially available substrates.
- Kit components
• ECL HRP Substrate Reagent A
• ECL HRP Substrate Reagent B.
- Features & Benefits
Abbkine SuperLumia ECL HRP Substrate Kit is a value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
- Usage notes
1.Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution. 2.Suggest 0.1-0.2ml of SuperLumia ECL working solution for 1 cm2 size of blot membrane. 3.Incubate blot 1-5 minutes in SuperLumia ECL Substrate working solution. 4.Drain excess reagent. Cover blot with clear plastic wrap. 5.Expose blot to X-ray film.
- Storage instructions
Stable for one year at 4-8°C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.
Gel pack with blue ice.
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
The family of SuperLumia ECL substrates is a series of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. SuperLumia ECL Chemiluminescent HRP Substrate Reagent is specially optimized for picogram amounts of antigen detection on immunoblots, with pouring directly onto the blot membrane without worrying about pipetting error. The substrate's intense signal output and duration enables detection of picogram amounts of antigen with photographic or other imaging methods perfectly well.
- Alternative names
Enhanced Chemiluminescence; ECL; Chemiluminescence; Electrochemiluminescence
Most popular with customers
SuperLumia ECL HRP Substrate Kit
Click to download booklet for more details
Notice:Protocol may vary from different lot which is subject to the manual along with the shipment. Please contact email@example.com for your enquiry.
Here we provide some standard research protocols for bioscience including molecular biology, cell biology, immunology, plant biology, genetics, etc. To our knowledge, customized protocols are not required for most products. So please try the standard protocols listed below and let us know how you get on.
Preparation methods for Biochemical
Biochemical reagents have been widely used in life science fundamental research as buffer, probes, substrates, intermediates and standards, etc. You may optimize or choose proper protocols for your specific assay. However, some of tips and suggestions listed below may be for your reference.
Antibody application protocols
Antibodies are useful not only to detect specific biomolecules but also to measure changes in their level and specificity of modification by processes such as phosphorylation, methylation, or glycosylation. Here show some protocols and troubleshooting tips on how to get the best from our antibodies.
- ♦ Antibody Western Blotting (WB) protocol
- ♦ Antibody Immunohistochemistry (IHC) protocol
- ♦ Antibody Immunofluorescence (IF) protocol
- ♦ Antibody Immunoprecipitation (IP) protocol
- ♦ Antibody Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol
Protein&peptide usage suggestions
Synthetic peptides, native or recombinant proteins can be used for medical, academic and research purposes, such as gene therapy, drug screening, antibody production, cell function analysis. Here, we provide some of tips and suggestions for your reference.
- ♦ Handling and storage suggestion for peptides and protein
- ♦ Cytokines and growth factors for cell culture application
Commonly used assay kits guidelines
Assay kits that are simple and convenient to use, which are superior in performance and require little to no time for assay optimization. Further details of specific products which are needed for individual protocols are given in the protocols themselves in booklet.
We hope this will be helpful for your research work. Please let us know through firstname.lastname@example.org if you need more information or support.
High-glucose induces tau hyperphosphorylation through activation of TLR9-P40MAPK pathway
Sun Y, Xiao Q, Luo C, et al. Exp Cell Res, 2017, 15; 359(2): 312-320.