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SOD2 Polyclonal Antibody

SOD2 Polyclonal Antibody

Views(773) Publications(0) Catalog no(ABP57228)
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Specification

Product name SOD2 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human SOD-2
Host Rabbit
Reactivity Human, Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative SOD2; Superoxide dismutase [Mn], mitochondrial

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background SOD2 (superoxide dismutase 2, mitochondrial) is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in SOD2 have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of SOD2 results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.
Gene ID 6648
Alternative SOD2; Superoxide dismutase [Mn], mitochondrial
Others SOD-2 Polyclonal Antibody detects endogenous levels of SOD-2 protein.
Accession P04179
Observed Band(KD) 22

Image & description

Fig.1. Western blot analysis of 1) 293T, 2) Hela, 3) C2C12,  4) 3T3, 5) Rat Liver Tissue, 6) Rat Brain Tissue using SOD2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western blot analysis of 1) 293T, 2) Hela, 3) C2C12, 4) 3T3, 5) Rat Liver Tissue, 6) Rat Brain Tissue using SOD2 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using SOD2 Polyclonal Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using SOD2 Polyclonal Antibody.

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