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SOD1 Polyclonal Antibody

SOD1 Polyclonal Antibody

Views(345) Publications(0) Catalog no(ABP57227)
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Specification

Product name SOD1 Polyclonal Antibody
Immunogen Synthesized peptide derived from the Internal region of human SOD-1
Host Rabbit
Reactivity Human, Mouse, Rat
Applications IHC-P, WB
Applications notes Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300). Not yet tested in other applications.
Clonality Polyclonal
Preparation method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
Alternative SOD1; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1

Product Properties

Formulation Liquid solution
Concentration 1 mg/ml
Storage buffer PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage instructions Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The protein encoded by SOD1 (superoxide dismutase 1, soluble) binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in SOD1 have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for SOD1.
Gene ID 6647
Alternative SOD1; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; hSod1
Others SOD-1 Polyclonal Antibody detects endogenous levels of SOD-1 protein.
Accession P00441
Observed Band(KD) 18

Image & description

Fig.1. Western blot analysis of 1) Hela, 2) MCF7, 3) Mouse Brain Tissue, 4) Rat Brain Tissue using SOD1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.1. Western blot analysis of 1) Hela, 2) MCF7, 3) Mouse Brain Tissue, 4) Rat Brain Tissue using SOD1 Polyclonal Antibody. Secondary antibody (catalog#: A21020) was diluted at 1:20000.

Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using SOD1 Polyclonal Antibody.

Fig.2. Immunohistochemical analysis of paraffin-embedded Mouse Kidney Tissue using SOD1 Polyclonal Antibody.

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