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Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit

Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit

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Specification

Product name Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit
Reactivity Rat
Applications ELISA
Applications notes This Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit employs a two-site sandwich ELISA to quantitate ZP2 in samples. An antibody specific for ZP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method Colorimetric
SampleType Cell culture supernatants, Other biological fluids, Plasma, Serum
Assay type Sandwich ELISA (quantitative)
Assay duration Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Alternative ZP2; ZPA; zona pellucida glycoprotein 2; zona pellucida protein A; zona pellucida sperm-binding protein 2

Product Properties

Kit components • Rat Zona pellucida sperm-binding protein 2 microplate
• Rat Zona pellucida sperm-binding protein 2 standard
• Rat Zona pellucida sperm-binding protein 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ZP2. No significant cross-reactivity or interference between Rat ZP2 and analogues was observed.
Calibration range Please inquire
Limit of detection Please inquire
Usage notes • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping Gel pack with blue ice.
Precautions The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Additional Information

Background The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. Zona pellucida sperm-binding protein 2 is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.
Alternative ZP2; ZPA; zona pellucida glycoprotein 2; zona pellucida protein A; zona pellucida sperm-binding protein 2
Accession P48829

Image & description

Fig.1. Rat Zona pellucida sperm-binding protein 2 (ZP2) Standard Curve.

Fig.1. Rat Zona pellucida sperm-binding protein 2 (ZP2) Standard Curve.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

Fig.2. Abbkine ELISA kit is series of sandwich ELISA to quantitate specific protein in samples.

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